In <4b44n0$bfa at news.fhg.de> Meike Mueller <mmueller at ita.fhg.de> writes:
>I have to prepare lymphocytes from popliteal lymph nodes. Does anyone
>know a useful method to get enough cells from these lymph nodes. I got
>from 10.000 up to 100.000 cells per lymph node. My problem is, I need 2.6
>10exp6 cells for my experiments.
Any particular reason to use popliteal lymph nodes? I assume you are
using naive mice, becuase when I immunize in the footpad with Freund's
I get huge nodes with 1-2x10^7 cells per animal. If you need cells from
an unimmunized animal can you try the mesenteric lymph nodes? Bigger
and easier to get out; more cells.
--
Tom Thatcher | You can give a PC to a Homo habilis,
University of Rochester Cancer Center | and he'll use it, but he'll use it
ttha at uhura.cc.rochester.edu | to crack nuts.
