fixation-sensitive antibody

username at bay.cc.kcl.ac.uk username at bay.cc.kcl.ac.uk
Thu Dec 21 13:55:14 EST 1995


I am a developmental biology student doing
whole-mount in situs with an antibody that is 
sensitive to most types of fixation methods.
At the moment it seems to be happy with a methanol/
DMSO fix, however this means that my embryos are 
undergoing massive shrinking during the protocol, and
we are concerned that the unfolding and refolding 
of all the proteins around is resulting in a matrix 
that is partially impenetrable to the antibody.
Any ideas would be greatly appreciated.

ZDBR336 at cc.kcl.ac.uk  Chris Blagden



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