Info on culture of human monocytes

clement clement at cvlab.harvard.edu
Fri Dec 22 13:08:43 EST 1995


Hello, 

I have initiated a project attempting to generate monocyte macrophages 
by cell culture of human blood monocytes. I am using Ficoll-paque 
gradients and adhesion to plastic tissue culture flasks for purification 
of monocytes.  These steps seem to work reasonably well, but the cell 
begin to detach upon further culture in RPMI-1640 media with 5% FCS, 
streptomycin and penicillin.  Are these conditions appropriate? How long 
does the monocyte to macrophage transition take? 

If any one has information on these questions or related information, 
please Email me at Fitzgerald at cvlab.harvard.edu  The above address is no 
longer current 





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