Hello,
I have initiated a project attempting to generate monocyte macrophages
by cell culture of human blood monocytes. I am using Ficoll-paque
gradients and adhesion to plastic tissue culture flasks for purification
of monocytes. These steps seem to work reasonably well, but the cell
begin to detach upon further culture in RPMI-1640 media with 5% FCS,
streptomycin and penicillin. Are these conditions appropriate? How long
does the monocyte to macrophage transition take?
If any one has information on these questions or related information,
please Email me at Fitzgerald at cvlab.harvard.edu The above address is no
longer current
