Problems in T cell proliferation assay

Maia Kuprianova maia at ibch.siobc.ras.ru
Wed Dec 27 11:57:43 EST 1995


Hello,
I am trying to do a T cell proliferation assay. Although T cells are growing, there 
is no radioactivity count after addition of 3H-thymidine. Why? Can the specific activity 
of 3H-thymidine make a difference and what is the optimum of it? Thank you very 
much.

Maia Kuprianova
maia at ibch.siobc.ras.ru




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