Dear fellow researchers,
I would be bery grateful if anyone could point to me an article
illustrating the PRINCIPLES of the immuneprecipitation reaction. It was
to date impossible to me NOT to find a protocol (there are at least one
for any immunology lab manual, and in the same book, without any reasons
the protocols may vary in two different chapters) but to find explained
WHY you should use a particular buffer,WHAT the advantages or
disadvantages of using protein A coupled to agarose instead of Ab linked
to sepharose as precipitin etc, etc, etc.
Thank you very much for any help.
Giorgio Spagnol