Affigel chromatography

kkatrak at nibsc.ac.uk kkatrak at nibsc.ac.uk
Mon Feb 6 10:24:58 EST 1995


I'd like some help regarding elution buffers for
purification of glycosylated proteins on antibody
coupled affigel-10 columns. The literature says use acid elution or base elutelution or chelating agents, so which is ideal?
I only have small amounts of precious material, so
can't afford to lose any.  Many thanks,





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