elispots

Idur idur at aol.com
Mon Feb 6 19:09:30 EST 1995


Daniela,

Maybe your second step antibody is not specific for the subclass. Another
option is, that your antigens do not bind very well to the carrier, and
IgM recognizes those trace amounts (multipoint attachment), but IgG does
not.
Another option is, that you do not have IgG antibodies at all.
Suggestions:
*You could address these issues by having a positive control to prove,
that you have antigen on the matrix. 
*You could establish the specificity of your conjugate to IgG by directly
coating mouse IgG, and looking for positive signal with your conjugate.



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