Help : Myosin Elisa

Robert C. Newton Robert_C._Newton at
Wed Feb 8 10:57:04 EST 1995

>We are used to doing ELISAS but are having big problems with myosin
>ELISAs. We get high backgrounds with binding of the second antibody
>anti-IgG. Has anyone any experience with myosin or special tips for
>cutting down backgrounds?

Large proteins (especially cytoskeletal) tend to be sticky.  How have you
formatted your assay (ex., capture, direct binding to mysoin coated plates)
and how have you isolated the second antibody as the problem?

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