Peptide solubility problems

Jane Goodall GoodallJC at bham.ac.uk
Thu Feb 9 07:29:17 EST 1995


Help! I am performing MHC/peptide binding assays by FACS but having
terrible problems with peptide insolubility following HPLC purification
(elutes @ 30% acetonitrile) and freeze-drying (sequence biotin-GVEGSLIVEKIMQSS).
 I have tried dissolving in PBS, DMSO,and acetonitrile. Initial dissolving is in
Guanadinium prior to HPLC, but this doesn't agree with cell culture (20h incubation
with EBVs). Any suggestions?
Thanks!

Jane.

p.s. a similar peptide biotin-GLEPGVVAEKVRNLS is no bother at all. Ho hum.



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