Help : Myosin Elisa

Frank C Hay frank at
Fri Feb 10 05:20:41 EST 1995

In article <310247423.82922146 at>, Robert_C._Newton at wrote:

> Large proteins (especially cytoskeletal) tend to be sticky.  How have you
> formatted your assay (ex., capture, direct binding to mysoin coated plates)
> and how have you isolated the second antibody as the problem?

We are coating the plates directly with myosin. The second antibody binds
to these coated plates and gives one of our problems, We have checked for
IgG in our myosin, but this does not seem to be the problem.

Adding normal serum also increases the problem.

Any help will be appreciated

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