Help : Myosin Elisa

[Frank C Hay]

In article <310247423.82922146 at dmpcfc.com>, Robert_C._Newton at dmpcfc.com wrote:


> Large proteins (especially cytoskeletal) tend to be sticky.  How have you
> formatted your assay (ex., capture, direct binding to mysoin coated plates)
> and how have you isolated the second antibody as the problem?
> 
> 
Robert

We are coating the plates directly with myosin. The second antibody binds
to these coated plates and gives one of our problems, We have checked for
IgG in our myosin, but this does not seem to be the problem.

Adding normal serum also increases the problem.

Any help will be appreciated