Adherent NK cells: No trypsin please!

[Rebecca Schall]

>Dear bionet.immunology readers,
>Can anyone suggest or know of a method for removing cultured 
>adherent NK cells from a plastic surface?  I don't want to use trypsin 
>or a cell scraper because I prefer the cells to be intact.  I've tried 
>using Teflon bags (American Fluoroseal Co.) for NK cell cultures ( all 
>cells are suspended), however the bags are cumbersome to work 
>with.
>M

I was of the understanding that adherent NORMAL NK populations lost 
their adherence rather quickly (24-72 hours), yet retained the 
phenotype of NK/LAK cells - see the publications of John Hiserodt of 
the early '80's on ALAK culture and purification.
Hope this helps-
Rebecca

Rebecca P. Schall, Ph.D.
PanVera Corporation
565 Science Dr.
Madison, WI 53711
(608) 233-9450
rebeccas at panvera.com