Adherent NK cells: No trypsin please!
REBECCAS at PANVERA.COM
Wed Feb 15 14:59:26 EST 1995
>Dear bionet.immunology readers,
>Can anyone suggest or know of a method for removing cultured
>adherent NK cells from a plastic surface? I don't want to use trypsin
>or a cell scraper because I prefer the cells to be intact. I've tried
>using Teflon bags (American Fluoroseal Co.) for NK cell cultures ( all
>cells are suspended), however the bags are cumbersome to work
I was of the understanding that adherent NORMAL NK populations lost
their adherence rather quickly (24-72 hours), yet retained the
phenotype of NK/LAK cells - see the publications of John Hiserodt of
the early '80's on ALAK culture and purification.
Hope this helps-
Rebecca P. Schall, Ph.D.
565 Science Dr.
Madison, WI 53711
rebeccas at panvera.com
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