Adherent NK cells: No trypsin please!

Martin Sorich msorich at
Wed Feb 15 02:23:37 EST 1995

Dear bionet.immunology readers,

Can anyone suggest or know of a method for removing cultured adherent NK 
cells from a plastic surface?  I don't want to use trypsin or a cell 
scraper because I prefer the cells to be intact.  I've tried using 
Teflon bags (American Fluoroseal Co.) for NK cell cultures ( all cells 
are suspended), however the bags are cumbersome to work with.


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