Adherent NK cells: No trypsin please!

[Martin Sorich]

Dear bionet.immunology readers,

Can anyone suggest or know of a method for removing cultured adherent NK 
cells from a plastic surface?  I don't want to use trypsin or a cell 
scraper because I prefer the cells to be intact.  I've tried using 
Teflon bags (American Fluoroseal Co.) for NK cell cultures ( all cells 
are suspended), however the bags are cumbersome to work with.

M