NK Assay

nuusers nubenne at nurs.indstate.edu
Wed Feb 15 15:52:31 EST 1995

I am working on a study which uses the standard CR51 release NK assay as an 
outcome measure. The intervention is probably going to produce a very small 
effect size, and I (and my advisor) am concerned that the day to day 
variability in the NK assay itself may obscure any effect from the 
intervention. It has been suggested that we try freezing the K562 target 
cells in lots at the start of the study, to attempt to syncronize the 
growth cycle of the targets to reduce variability related to target cell 
changes in culture. Does anyone out there have any information concerning 
NK assay reliability or use of the suggested method?

Mary Bennett
Nubenne at nurs.Indstate.Edu
Indiana State University

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