Adherent NK cells: No trypsin please!

[Jorg Kirberg]

In article <3hsa5p$fta at ixnews2.ix.netcom.com>, msorich at ix.netcom.com
(Martin Sorich) wrote:

> Dear bionet.immunology readers,
> 
> Can anyone suggest or know of a method for removing cultured adherent NK 
> cells from a plastic surface?  I don't want to use trypsin or a cell 
> scraper because I prefer the cells to be intact.  I've tried using 
> Teflon bags (American Fluoroseal Co.) for NK cell cultures ( all cells 
> are suspended), however the bags are cumbersome to work with.
> 
>

Dear Martin,

at least for fibroblasts, which adhere very strong to tissue culture
plates, I used to put them after trypsinisation for 1 - 2 days on
plastic bacterial dishes. As these are not tissue culture treated the
fibroblasts (Ltk- i used) could not adhere, They could simply be 
washed away by forcefully using a pasteur pipette.

jorg

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Joerg Kirberg                     EMAIL: kirberg at bii.ch
Basel Institute for Immunology    FAX:   41-61-605 13 72
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