Adherent NK cells: No trypsin please!

Ed Purcell epurcell at
Fri Feb 17 10:03:54 EST 1995

In article <3hsa5p$fta at>, msorich at (Martin Sorich) says:
>Dear bionet.immunology readers,
>Can anyone suggest or know of a method for removing cultured adherent NK 
>cells from a plastic surface?  I don't want to use trypsin or a cell 
>scraper because I prefer the cells to be intact.  I've tried using 
>Teflon bags (American Fluoroseal Co.) for NK cell cultures ( all cells 
>are suspended), however the bags are cumbersome to work with.

Sigma has a nonenymatic cell dissociation solution to remove cells from 
plates that we have had some success with for macrophages.

Ed Purcell
University of Osteopathic Medicine and Health Sciences, Des Moines, Iowa
epurcell at

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