help in transiently transfect cultured B- and T- cell lines

Gavin Fischer gfish at bu.edu
Fri Feb 17 13:29:48 EST 1995


References: <D3FFMp.6K at acsu.buffalo.edu>

While in David Scotts lab we used Electroporation and DOTAP (Boeringer)
to transfect murine lymphomas (WEHI231 and CH31) both transiently and
stablly (sp?). Settings on the Electoporator were 250 V and 960 micro
farads. We didn't do anything with primary cells while I was there.
As for the antisense, was done in lymphomas as well, and 
gives a wide variety of effects. 


Gavin Fischer
Boson University
gfish at acs.bu.edu



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