Dear Netters,
I plan to raise polyclonal antibodies to a eukaryotic
protein expressed in E. coli. I plan to do this by injecting the rabbit with
an emulsion of the protein in freunds complete adjuvant. Will the fact that the
eukaryotic protein was expressed in E. coli be of any hinderance to formation
of antibody which recognize structural epitopes( due to not proper folding in
the E. coli) ? I will be great full for any comments on this. thanks in
advance.
