REQUEST: Dose of KLH for DTH immunization of rat

Dr Immuno drimmuno at
Sat Feb 18 23:59:25 EST 1995

I would appreciate some help for a collegue of mine in Hampton, VA.  He
wants to generate a good delayed type hypersensitivity response aginst
Keyhole Limpet Hemocyanin in male Sprague-Dawley rats.  He needs a dose
that will give a strong, but suboptimal delayed hypersensitivity response
14 days after a single immunization.  What dose and route should he use
for immunization?  Should he use adjuvant?  If so, which adjuvant? 
Finally, what dose should he use to challenge the rats intradermally and
in what volume.  If anyone has a methods book with this information in it,
I would be grateful...Dr. James Wise - Hampton University

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