Hybridoma production failure

[ANDREI POPOV]

>Recently we failed to grow hybridoma cells.  It appeared that the myeloma
>cells were proliferating at the expense of the hybridomas.  What is the
>most likely explanation?  Is the aminopterin suspect?  Can SP2/0 myeloma
>cells become aminopterin-resistant?  The cells that did grow appeared
>earlier and grew faster than expected and failed to secrete antibody.  Any
>ideas?

 
Hi
I would test my myeloma on HAT containing medium.
Normally SP2/0 or NS0 cells should dye in 3-4 days.
They might undergo 1 division after application of HAT.
Nobody, to my knowledge, has reported revertants in SP2/0.
                               
Good luck
Andrei