Hybridoma production failure
ANDREI.POPOV at afrc.ac.uk
Thu Feb 23 18:45:02 EST 1995
>Recently we failed to grow hybridoma cells. It appeared that the myeloma
>cells were proliferating at the expense of the hybridomas. What is the
>most likely explanation? Is the aminopterin suspect? Can SP2/0 myeloma
>cells become aminopterin-resistant? The cells that did grow appeared
>earlier and grew faster than expected and failed to secrete antibody. Any
I would test my myeloma on HAT containing medium.
Normally SP2/0 or NS0 cells should dye in 3-4 days.
They might undergo 1 division after application of HAT.
Nobody, to my knowledge, has reported revertants in SP2/0.
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