Need rat antimouse CD8 mAb, YTS 169.4, or other mAbs to eliminate CD8s in adult mice.
Michael Newton Robertson
newton at saul
Sun Feb 26 13:41:42 EST 1995
Michael N. Robertson, MD Telephone (206) 685-3666 FAX 685-3639
Department of Medicine, Division of Infectious Diseases, SC-42
Retrovirus Lab, Room T-293A, University of Washington Medical Center
1959 NE Pacific St, Seattle, WA 98195 USA
On 25 Feb 1995, Agnieszka Szczepek wrote:
> On 24 Feb 1995, Aki Hoji wrote:
> > Hi all !!
> > I am planning to eliminate CD8+ cells in adult mice, and I need a
> > monoclonal YTS 169.4 which is the rat anti mouse CD8 mAb. Since I don't
> > quite know exactly where to obatin this mAb, or the hybridoma producing it
> > (meaning, it is not commercially avialable), any help will be appriciated
> > to locate it. Also, I like to know if there are any other mAbs avialable
> > to do the same job. Thanks in advance.
> You can buy the rat-anti-mouse CD8 Abs from Pharmingen (1-800-848-MABS);
> they have two types of Abs- one against the alpha chain of CD8 (Ly-2) and
> other one against the beta chain of CD8 (Ly-3.2).
Note that these antibodies may not work for in vivo elimination of CD8
cells. It depends on the subtype of the antibody. When antibodies YTS
169.4 and its related anti-CD4 antibody were derived (see Nash, et al J.
Gen. Virol., vol 68, page 825-833) only certain subtypes worked for in
vivo depletion. Another antibody is 2.43 (see Sarmiento, et al, J.
Immunol. vol 125, pp 2665-2672). I suspect the Mab from Pharmigen were
not selected for use in in vivo depletions. I got YTS 169.4 from Michael
Oldstone's lab a number of years ago and 2.43 from Frank Fitch's lab.
This was because my boss at that time called these guys up and they sent
it. I don't know if they will give them to anybody, but they may know a
source. I believe Fitch originally derived 2.43.
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