non-radioactive proliferation assay

[rsorg at chmeds.ac.nz]

Dear netters,

I am looking for a non-radioactive system to replace [3]T uptake in
proliferation assays. Sofar, I tried MTT and Alamar Blue (PHA+IL-2 stimulated
PBMC) but I was not happy with the results. For Alamar Blue, the signal/back-
ground ratio was pretty bad, for MTT, I had the same problem and in addition 
a wide variation of the ODs. Are there better systems? Any comment would be 
appreciated.

Ruediger


 
Ruediger V. Sorg, Ph.D.            |
Christchurch School of Medicine    |
Haematology/Immunology Research    |
Christchurch, New Zealand          |
E-MAIL RSORG at CHMEDS.AC.NZ          |
PHONE #64 (3) 364-0553             |
FAX   #64 (3) 364-0750             |