Staining for BUdR & CD4

macpherson at molbiol.ox.ac.uk macpherson at molbiol.ox.ac.uk
Wed Jan 18 07:24:31 EST 1995


We are having trouble with double staining for BudR and CD4 on rat cells.  If 
we hydrolyze with HCl first, the CD4 epitpope is destroyed (we have titrated 
hydrolysis down to the minimum).  If we stain first for CD4, the subsequent 
hydrolysis destroys the diaminobenzidine precipitate.
	If anyone has a reliable protocol, or can ppoint me in the right 
direction, I would be most grateful.

Gordon MacPherson
macpherson at molbiol.ox.ac.uk



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