bact. alkaline phosphatase

Torsten Borchers borcher at asterix.uni-muenster.de
Sat Jan 28 13:56:45 EST 1995


Dear immuno netters,
as this appears to be a newsgroup also devoted to practical questions
I would like to ask the following:

Alkaline phosphatase is one of the most frequently used marker enzymes
in ELISA applications. Most of the recipes for preparation of conjugates
I am aware of use calf intestine alkaline phosphatase. Has anybody 
experience with bacterial alkaline phosphatase for this purpose? 
Is this enzyme - at least sometimes - used?

I know that bact. AP has lower activtiy but there is also some mess
with unit definitions.

Any information is appreciated

Yours

Torsten 
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Torsten Boerchers
Institute for Chemical- & Biochemical Sensor Research
Mendelstr. 7, D-48149 Muenster, Germany
Phone +49-251-980 2876, Fax +49-251-980 2890
borcher at uni-muenster.de
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