Culture of lymphocytes

[In C. Kim]

Cell culture is performed generally in a humidified, CO2-incubator.  In
order to prepare metaphase spreads, lymphoyctes are cultured for only
three days in the presence of a mitogen and colicimide.  I wonder if this
condition requires CO2 or not.  Could anybody have experience in culturing
human peripheral lymphocytes for genetic research? 


--In C. Kim   <inkim at carina.unm.edu>