Culture of lymphocytes

In C. Kim inkim at
Fri Jan 27 08:49:59 EST 1995

Cell culture is performed generally in a humidified, CO2-incubator.  In
order to prepare metaphase spreads, lymphoyctes are cultured for only
three days in the presence of a mitogen and colicimide.  I wonder if this
condition requires CO2 or not.  Could anybody have experience in culturing
human peripheral lymphocytes for genetic research? 

--In C. Kim   <inkim at>    

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