Flow cytometry

DANDERSON at PRL.PULMONARY.UBC.CA DANDERSON at PRL.PULMONARY.UBC.CA
Thu Jul 20 09:26:09 EST 1995


immunodoc at aol.com wrote:

>I wonder if you're seeing binding of the antibodies to Fc receptors. What is
>the form of your secondarys?  Fab2? If so, Fab2 fragments of your primary
>antibodies or blocking of the Fc receptors with normal Ig might help.  Good
>luck.

Thanks for your response.  

I have tried both blocking with species specific normal serum and with 
an Fc blocking antibody.  The non-specificity with the rabbit 
anti-virus series is in the primary as my donkey anti-rabbit has no 
background staining.

With the murine anti-virus MAb the secondary Ab sticks with goat normal 
serum in the block and in the primary cocktail and with the Fc block.

Dan



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