> I am trying to work out a method where I could
> run a DNA sample on an electrophoresis gel, and
> then cut out a well adjacent to the lane where
> the DNA is and load it with antibody, converting
> the electrophoresis gel to an ouchterlony. Does
> anyone know if this is possible? The buffer
> I wish to use is TAE. Can this be used for both?
> If not is there another buffer that can be used?
> I would appreciate any feed back. Thanks in
> advance.
>> Sincerely,
>> Tammy J. Rossi
> Clinical Research Specialist II
>>Hi, what do you want exactly? DNA is not protein so how can you want
to use it as an antigen in agar gel precipitation test(ouchterlony)
I think if you have antibody raised against DNA you may set SDS-PAGE
running for your DNA, transfer it to a solid phase and you may now
able to do immunodetection. Or you may extract your DNA from agarose
in TAE using Qiaex protocol, then you can do ouchterlony .
Please tell me the results after your trial.
>
