Help! I am a grad student at the university of Dayton, Ohio, working on
pyocyanin. This is a pigment which is produced by Pseudomonas aeruginosa. I
have been working with it for almost 2 years, and I am not sure that what I
have is actually pyocyanin. I have been conducting humoral immunity studies
(hemolytic plaque assays) with this compound and they are not giving me the
results that are expected from the literature. I think the problem may be
in the compound I have and not the techinique as I have managed to
standardize it fairly well. If anyone is working with pyocyanin of knows
someone else who is, please contact me. I would like to possibly exchange
notes on how this pigment is purified, or better yet, perhaps get a small
sample to test against my pyocyanin. Thank you.
Angela M. Gallo
e-mail: Galloanm at udavxb.oca.udayton.edu
