Cleavage of MHC class II by trypsin

Ken Frauwirth BioKen frauwirt at notmendel.Berkeley.EDU
Mon Jul 31 15:23:28 EST 1995


In article <199507311843.NAA04442 at wugate.wustl.edu>,
Kim Sabelko <ksabelko at PHARMDEC.WUSTL.EDU> wrote:
>Netters,
>
>Does anyone know if trypsin can cleave MHC class II molecule off of cell
>membranes.  
>I am trypsinising my adherent APC to get them off the plate and I wonder if
>trypsin affects class II at all.  Please help.
>
>Thanks.

It is my understanding that MHC Class II is pretty trypsin-resistant (I've 
used trypsin on cells for FACS and detected decent amounts of A(k) ).  If
you are concerned, you can try just using EDTA to lift the cells (many 
adhesion molecules require divalent cations to work).

Good luck,

BioKen
-- 
Ken Frauwirth (MiSTie #33025)                         _           _
frauwirt at mendel.berkeley.edu                         |_) *    |/ (_ |\ |
http://mendel.berkeley.edu/~frauwirt/HomePage/       |_) | () |\ (_ | \|  
DNRC Title: Chairman of Joint Commission on In-duh-vidual Affairs



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