jog1111 at aol.com
Thu Jun 1 11:15:29 EST 1995
Matthew, Bovine IgG contamination is a constant problem. I'm not sure
about Prot G , it will depend on the relative affinities of the bovine
vs. your product. A and G are worth a try. Max load the resin and run
very slowly (20 cmhr) If the G4 affinity is higher it may help to displace
the bIgG. Also, I have had success in the past using HIC resins to
separate Product IgG from Bovine using very small difference in salt conc.
(2-5%). Good luck.
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