IgG purification

JOG1111 jog1111 at aol.com
Thu Jun 1 11:15:29 EST 1995


Matthew,  Bovine IgG contamination is a constant problem.  I'm not sure
about Prot G ,  it will depend on the relative affinities of the bovine
vs. your product.  A and G are worth a try.  Max load the resin and run
very slowly (20 cmhr) If the G4 affinity is higher it may help to displace
the bIgG.  Also, I have had success in  the past using HIC resins to
separate Product IgG from Bovine using very small difference in salt conc.
(2-5%).  Good luck.
                                                                   John



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