antibody at bii.ch
Fri Jun 2 11:23:46 EST 1995
In article <852 at kiwi.ukc.ac.uk>, mrr1 at ukc.ac.uk (M.R.Reece-Ford) wrote:
> I have a CHO cell line that produces a chimeric IgG4 that unfortunately has
> to be cultured in serum containing media (8% feotal calf serum). This FCS also
> contains bovine IgG which I would like to selectively remove from the culture
> supernatant. Has anyone had any experience with this problem? I have read that
> Pharmacia's Protein G Fast Flow 4 will remove the bovine IgG has anyone tried
> it? Finally how about purifying the FCS before addition to the media, is this
> feasible or would maintaining sterility of the serum be to difficult (the FCS
> has components that would be denatured if autoclaved). Thanks in advance.
in our lab, FCS is passed over the affinity column first, then sterilized
by filtration again (0.2 micom) before adding to the cultures.
More information about the Immuno