Western blotting Pan Class I mAb

Mike Whelan whelan at bbsrc.ac.uk
Mon Jun 12 04:57:48 EST 1995


Hello Shane,

It's Mike here. Found your post on the Internet Immunology group. I think 
the answer you are looking for is Hidde Ploegh's HC-10 MoAb. He is 
usually very good about giving it out and there is a stock at the ICRF.

He also uses a rabbit polyclonal serum to do the same job. In the 
original Stam paper they never actually say how the stuff was raised but 
I suspect it has something to do with SDS extracts since you can 
immunoprecipitate these with it.

If you need more help get in touch.

Mike Whelan
IAH




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