Lymphocyte proliferation tests

FORSDYKE at QUCDN.QueensU.CA FORSDYKE at QUCDN.QueensU.CA
Thu Jun 15 16:05:40 EST 1995


    Try measuring total RNA. It is very cheap and could be used in the "third
 world". All you need is a bottle of NaOH, a bottle of perchloric acid and
 access to a uv spectrophotometer (hmmm. not so cheap!). The reference is
 Biochem. J (1967) 105: 679 "Quantitative nucleic acid changes during PHA-
 induced lymphocyte transformation in vitro."
                                             Sincerely,
                                             Don Forsdyke,
                                             Discussion Leader. Bionet.Immunol.



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