TNFa-anyone sequenced frag. amplified by primers for rat??

Mary P. Remington mremingt at umabnet.ab.umd.edu
Fri Jun 16 08:38:37 EST 1995


We are using the Clontech Rat TNF-alpha primers to amplify TNF in rat.  
The GCG sequence indicates that the primers supplied by Clontech should 
amplify a fragment ~570 bp while Clontech says the product should be 295bp.
The actual fragment size is 295bp but, why is there a discrepancy?  It is 
difficult to get any quick answers from Clontech on this issue.  Takes a 
couple of days to hear back from tech services.  Thanks for any input.  Mary





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