In vitro Priming of CTL

David Peritt Peritt_d at a1.mscf.upenn.edu
Mon Jun 19 07:32:51 EST 1995


In article <gpier-1606951804280001 at 134.174.147.50> Gerald Pier,
gpier at warren.med.harvard.edu writes:
>Concanavalin A will activate CD8+ cells in vitro but it is a polyclonal
>activation so it will not select out for or induce CTL specific to any
>particular antigen.  There is a fair amount of literature on Con A
>activation of human T cells and its resultant effects.  A older (1977)
>reference for long term cultivation of CTL's is Gillis and Smith, Nature,
>268; 154-156.  I don;t know if this helps as you might be interested in
>"inducing" antigen-specific CTL in vitro, in which case you'll need to
dig
>deeper.

In our hands ConA is a great stimulator of murine cells but lackluster on
humal PBL.  We routinely use PHA for human work.  This gives us a great
polyclonal stimulation of CD8 and CD4 cells.



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