XTT is better

Jean-Pierre Scheerlinck scheerjp at wehi.edu.au
Mon Jun 26 17:56:59 EST 1995


David Peritt (Peritt_d at a1.mscf.upenn.edu) wrote:
: In article <Pine.SGI.3.91.950613205852.14851E-100000 at tower.york.ac.uk>
: Cosme Malaquias, cm111 at unix.york.ac.uk writes:
: >The MTT(3-[4,5 dimethylthuazol-2yl] 2,5-diphenyl) tetrazolium bromide) 
: > and can be purchased from sigma. You can use  10 ul to 100ul of 
: >final volume from a stock solution of 5 mg/ml in PBS.

: solubilization is always a problem.  Try an aqueous form called XTT
Solubilisation of MTT is possible by adding 100 µl (i.e same volume as is
already in the well) of a solution containing 50g of SDS, 0.5 ml HCl and 500 ml
of H2O.
This is left overnight at 37 C. before the O.D is measured in an ELISA reader.

Jean-Pierre Scheerlinck.
>



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