Freezing of new hybridomas in a 96well plate

SBehar sbehar at aol.com
Wed Jun 28 21:50:37 EST 1995


I am interested in hearing about the experience of anyone who has frozen
freshly made hybridomas in a 96 well plate.

1) what is the best freezing protocol
2) what is the best way to thaw and recover the hybridomas
3) what is the typical recovery

Many people talk about doing this as if it is routine, but I have not met
anyone who has successfully used this to recover a reasonable yield of
hybridomas.  I need a way to preserve my fusion as I want to screen by
immunoprecipitation.

Thanks.
Samuel M. Behar, MD PhD
Seeley G. Mudd Bldg.-- Rm 508
Department of Rheumatology
Brigham and Women's Hospital
250 Longwood Avenue
Boston, MA 02115
FAX: 617-432-2799



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