Purifying antibodies by immunoblots

graethes at jeff-lab graethes at jeff-lab
Thu Jun 29 18:20:16 EST 1995


As the title says, we are thinking of purifying our antibodies by eluting 
them off of our immunoblots. We can't use the traditional 
antigen-affinity column, since we can't easily purify our antigen.
 
I would like to here what people have to think about this technique 
(yields, specificty). The problem with our IgG purified antibodies is 
that they have a natural cross reaction to another protein. We hope that 
this will allow us to get antibodies more specific to our antigen. 

I would also appreciate any other references on this technique than 
Olmsted (1981) and Fisher and ? (1984).

Steffen



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