eliza

vsem vsem%compsvc.FRC.MASS.EDU at MADMAX.FRC.MASS.EDU
Thu Mar 2 23:20:48 EST 1995


   hELLO!
  I have an interesting question.
  Say I have 2-fold serial dilution made of immunized serum.  50 uL of each
dilution were placed into the ELISA plates coated with excess antigen.
After a primary Ab wash and secondary anti-serum linked to substrate.  The
result are:

2+, 4+, 8+, 16+, 32+, 64+, 128+, 256+, 512+/-, 1024-,
2048-, 4096-, 8192-, 16384,.... for BSA
                and
2+, 4+, 8+, 16+/-, 32-, 64-, ...... for TCB (

Q:  Were there anti-BSA in the immunized blood?
"    "     "    anti-TCB "   '     "       "  ?
If so what are the titers of the antibodies?  And the approx. concentration?



                                Any help will be greatly appreciated!



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