rat TNFa cross react with human endo./or PMNs?

Yasuhito Abe yasuhito at ME1SERV.M.EHIME-U.AC.JP
Mon Mar 6 01:09:59 EST 1995


At 10:26 PM 95.3.5 +0000, Jeffrey Upperman wrote:
>does anybody know if rat tumor necrosis alpha cross reacts with human 
>endothelial cells or polymorphonuclear cells?
>
>thanks
>-- 
>Jeffrey S. Upperman,MD                 Internet: upperman at umdnj.edu
>Research Fellow                           Voice:  (201) 982-4474           
>Dept. Anatomy, Cell Biology & Injury        Fax:  (201) 982-6803
>Dept. of Surgery                         Beeper:  (201) 708-4150
>New Jersey Medical School                Office:  (201) 982-5045
>185 S. Orange Ave., G606                    Lab:  (201) 982-5404
>Newark, NJ 07103


Dear Sir;

        I think it does.  But you need attention and careful examination to
prove it.  Evidences ever elucidated are:
1.  Human TNF (TNF-alpha) binds to and triggers signals of 55-kDa but not
75-kDa mouse TNF-R.  Therefore, human TNF is weaker than mouse TNF in some
activities of mouse system.
2.  Mouse TNF binds to and triggers signals of at least 55-kDa human TNF-R.

According to abve these data, rat TNF is expected to bind and signal at
least 55-kDa human TNF-R, which works as the main signaling TNF-R as for
endothelial cells and PMN.  But, I am not sure if mouse TNF binds to 75-kDa
TNF-R or not. In addition, I don't think you can find out data on rat TNF
in recent articles.  Though it depend on your experiment system, you might
need not to clear these points.  Anyway, I think it does.  Try.

 
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