Precipitin zones

JAF at UNCVX1.OIT.UNC.EDU JAF at UNCVX1.OIT.UNC.EDU
Mon Mar 6 09:48:31 EST 1995


Try using buffer instead if water in your agarose.  For a detailed 
discussion see Chp 32 ofthe handbook of experimental immunology (Weir ed) 
blackwell 1986.
Jeff Frelinger

On Fri, 3 Mar 1995, Lewis X. Liu wrote:

> I needed suggestions on how to make precipitin zones visible to the naked eyes 
> in agar gels.  I know that antibodies reacted to its specific antigens forming 
> precipitin zones in agar gels (3% agar in water), but I have trouble making it 
> visible to the naked eye.  Is there any reagents that I could use to make 
> these precipitin zones visible (such as dyes, etc.)?  Any suggestions are 
> appreciated.  Thank you.
> 
>  
> 
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