yeast two hybrid cDNA libraries

ewoodward at gems.vcu.edu ewoodward at gems.vcu.edu
Tue Mar 7 10:30:21 EST 1995


I am looking for a mouse cDNA library from B cells that is in expression 
plasmids for use in the yeast two hybrid system - fused to either the GAL4 
activation domain or the B42 activation domain.  Does anyone have one or know 
of a non-commercial source?  I am also curious if anyone has any info on 
expression of extracellular domains of membrane bound proteins in the yeast 
two hybrid system.  I have seen reference to unpublished results using 
antibody-antigen interactions but no data was shown.  Thanks -
Elaine Woodward
ewoodward at gems.vcu.edu
Virginia Commonwealth University 
Richmond, VA 23298




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