MONOCLONAL ANTIBODIES AND ANTIBODY ENGINEERING

Let's Eat!!! hutton_rl at MRICD4.APGEA.ARMY.MIL
Fri Mar 10 16:40:42 EST 1995


> From:	SMTP%"zcbcl02 at ucl.ac.uk"    10-MAR-1995 15:55
> To:	immunology at net.bio.net
> CC:	
> Subj:	MONOCLONAL ANTIBODIES AND ANTIBODY ENGINEERING
> 
> FRIENDS,
> 
>        I've got a 5000 word project on " Monoclonal Antibodies and
> Antibody Engineering".  I'm a Molecular Biology student, do any of you
> immunological wizards have any ideas on where to start, and what to
> include?
> 
> 
>         THANKS
> 
> 
>    ADAM


	Well, your project is right up our alley!  I'm working with a group to 
produce catalytic antibodies to organophosphate transition state analogs.  
Right now, we're cloning monoclonal antibodies into pComb3H-SS, a plasmid 
developped at Scripps labs in La Jolla.  We plan on using a phage display 
technique with VCMS 13 as helper phage (for reference on this technique used in 
another context, you can see Barbas, et al.; Proc. Natl. Acad. Sci. USA Sept. 
1991).  We're currently also using capillary electrophoresis in conjunction 
with the regular agarose gel work to check ourselves along the way.  If you 
have anymore questions, write back and I'll do my best to help out!
	Please respond;  I'd like to see how your project turns out.

Robert L. Hutton
US Army Inst of Chem Defense
Edgewood, MD
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> From: zcbcl02 at ucl.ac.uk (Adam Assem Rostom)
> Subject: MONOCLONAL ANTIBODIES AND ANTIBODY ENGINEERING
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> Date: Fri, 10 Mar 1995 17:30:40 GMT
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