CD4 and HIV

Mario Roederer Roederer at Darwin.Stanford.Edu
Fri Mar 10 15:13:23 EST 1995


In article <3jonme$kc9 at lastactionhero.rs.itd.umich.edu>, Luis Diaz
<cascas at umich.edu> wrote:

> If one transfects human CD4 into a human CD4 negative non-hemopoetic
> cell, does this alone allow for HIV to infect these cells ?
> 
> Just curious 
> 
> Luis Diaz
> Graduate Student
> Univ. of Michigan

I have successfully infected (in fact, both acutely and chronically) a 293
clone in which I transfected a CD4 expression vector.  293 cells are
derived from human embryonic epithelial kidney cells transformed with
adeno.  The parental line is not infectable with HIV; the CD4+ line is
productively infectable.  (BTW, 293 is a somewhat unusual cell line, in
that it can be transiently transfected to >70% efficiency, and is
remarkably hardy...).  BTW, the infection did not seem to kill the cells.

I have not tried with other cell lines.

mr



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