Laurentiu COCEA previously wrote:
>I wonder if someone has ever tryied to count the minutes (hours? days?) you
>have to wait for an Ig to be expressed at the surface of a lymphocyte once
>transcription has started. It might be different for the H chain that
>associates with the lambda 5 protein and for a "normal" H/L protein.
>If the answer is negative, haw do you think this could be measured?
i'm sorry Laurentiu, that i don't know how long this takes, but i do
know that the jan 1994 issue of cell has a paper in it that refutes the l-5
and v-pre-b data and says that these "surrogates" are only expressed
_before_ mu, and that as soon as mu mRNA is made, surface expression of both
of these "surrogates" are gone. so it prob won't be diff for "the H chain
that associates with the lambda 5 protein and for a "normal" H/L protein",
since they don't. the surrogate data that said otherwise was from
transformed cell lines (viraly trans cells are wacky). mayhaps the
surrogates in b-cells play a role in early early b-cell + selection?
anythoughts on this?? anyone read anything new about surrogates???
Ralph
Ralph M. Bernstein
Dept of Micro/Immuno
University of Arizona
Ph: 602 626 2585
Fx: 602 626 2100
