Heelp! Please explain what this assay is!!!

David Peritt Peritt_d at a1.mscf.upenn.edu
Tue Mar 21 09:51:14 EST 1995


In article <3kmgg4$7tn at decaxp.harvard.edu> derek chan,
chan4 at fas.harvard.edu writes:
>I am interested in knowing how Ethidium Bromide/Acridine Orange can be 
>used to see DNA fragmentation (apoptosis) by staining cells and then 
>looking _at the cells_ under a microscope.  I am familiar w/ EtBr 
>staining of DNA and running it out on a gel, but I don't think this is 
>what's being described here...
>
>Please help.  

This is a method to visualize morphologically the DNA blebbing.  This was
used as early as the 60's by Wiley et al.  in their description of
apoptosis.  Today many more elegant techniques are available that are
easy to do and quantitate.  We use TUNEL which is superior to
hypochromosomal shifts with Propidium iodine or DNA laddering.



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