Immunoblotting and gels

Wayne R. Baker baker at iastate.edu
Thu Mar 23 16:19:32 EST 1995


In bionet.immunology,  Joel--Overland Park KS <guss at tyrell.net> wrote
:
:I am looking for some information that will basically describe to me how
:to run gels and how to blot and how they work.  One sourse that someone
:recommended was catalogs that sell kits for these things; even though they
:are just trying to sell it, they really do provide good explinations on
:how it works.  Does anyone have any more ideas?
:thanks in advance.

Most any book on Biochemistry or Biophysics will have a discussion of
gel electrophoresis and most Immunology and Molecular Biology books will
talk about blotting. "Molecular Cloning: A Laboratory Manual" by J.
Sambrook, E. F. Fritsch & T. Maniatis published by Cold Spring Harbor
Press is an excellent resource. Electrophoresis techniques are covered
in volume 3. 

Wayne Baker (baker at iastate.edu)		He told the truth mainly.  
4288 Molecular Biology Building  	There were things which he stretched
Iowa State University            	but mainly he told the truth.
Ames IA 50011                   	--Mark Twain
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