Reaction/mechanism for BCIP+NBT in immunoblotting

root root at
Fri Mar 24 22:15:44 EST 1995

In article <3kt7fb$1b22 at>, 
root at says...
>        In the reaction between 5-bromo, 3-chloro, 4-indoyl phosphate 
>suspension (BCIP) and nitroblue tetrazolium(NBT) which is catalyzed by the 
>enzyme alkaline phosphatase, what is the reaction/mechanism for this 
>reaction?  Does anybody know where I can find any journal references 
>to this reaction?
>                                Sincerely,
>                                     Undergraduate desiring xtra credit...
				      Paul Ferry, OSU

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