chicken B cell differentiation

Seth Corey corey at MED.PITT.EDU
Tue Mar 28 01:10:54 EST 1995


I am studying the DT40 cell line. and I wish to check if the cells
are differentiating in response to a growth factor. I can do this
by either protein or mRNA analysis. So far, I checked the soup to
see if chicken Ab is present. This many not be sensitive, and the use
of chicken serum will give a band.

any suggestions? cDNA probes? antibodies?

thanks,
seth corey



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