Wanted - Murine IL-10 cDNA construct

bwalker at comp.nibsc.ac.uk bwalker at comp.nibsc.ac.uk
Tue Mar 28 06:34:50 EST 1995


In article <3l6slo$4ai at crocus.csv.warwick.ac.uk>, lsrbd at csv.warwick.ac.uk (Chris Hodgson) says:
>
>Dear Immuno/ Molbio netters
>
>I'm in need of the cDNA for IL-10.


If you are trying to make riboprobes or ISH etc try making the probe from
a PCR product tailed with T7 polymerase promoter (the 3 prime pimer
having the T7 pol promoter added) .  There are a number of 
recent publications describing this procedure.  Its somewhat easier than
cDNA cloning etc - and works!

B-Walker




More information about the Immuno mailing list