In article <3l6slo$4ai at crocus.csv.warwick.ac.uk>, lsrbd at csv.warwick.ac.uk (Chris Hodgson) says:
>>Dear Immuno/ Molbio netters
>>I'm in need of the cDNA for IL-10.
If you are trying to make riboprobes or ISH etc try making the probe from
a PCR product tailed with T7 polymerase promoter (the 3 prime pimer
having the T7 pol promoter added) . There are a number of
recent publications describing this procedure. Its somewhat easier than
cDNA cloning etc - and works!
B-Walker
