In article <9502287964.AA796438065 at internet.organo.com>, MKing at organo.com
("King, Matt") writes:
>I am looking for any introductory info concerning flow cytometry, especially
>the use of a FACScan. I am beginning to perform research on class I and II
>antibodies and need to learn how to use the FACScan in our lab. I understand
>the priciple behind using it to a degree, but any literature you out there can
>suggest will help to strengthen my knowledge on the subject. Please help point
>me into the right direction.
>
Probably the best source to learn how to operate the FACScan is the
manufacturer. Contact Becton Dickinson Immunocytometry Systems (BDIS) at:
2350 Qume Drive
San Jose, CA 95131-1807
phone: 800-448-BDIS (800-448-2347)
A good introductory text, one that is used in teaching several university
courses on flow cytometry is:
Givan AL: Flow Cytometry: First Principles. John Wiley & Sons, Inc., New York,
1993.
A "cookbook"-style handbook of methods is:
Robinson JP (ed.): Handbook of Flow Cytometry Methods. 1990.
(The copyright is held by Purdue Research Foundation, but the handbook is
distributed by ISAC [see next]).
The Journal of the International Society for Analytical Cytology (ISAC), called
Cytometry, is a good resource. Send subscription inquiries to:
John Wiley & Sons, Inc., Subscription Department
9th floor
605 Third Avenue
New York, NY 10158
phone: 212-850-6543
Some more advanced texts are:
Melamed MR, Lindmo T, Mendelsohn ML (eds.): Flow Cytometry and Sorting, Second
Edition. John Wiley & Sons, Inc., New York, 1990.
Shapiro HM: Practical Flow Cytometry, Second Edition*. Alan R. Liss, Inc.,
New York, 1988.
*There is a newer Third Edition available now.
There is a mailing list devoted to flow cytometry. Contact Steven Kelley via
email at:
kelley at flowcyt.cyto.purdue.edu
A few web servers dedicated mostly to flow cytometry have sprung up recently.
The one at the Salk Institute has some tutorials/help files, as well as links
to most of the other cytometry web servers. Open the URL:
http://flosun.salk.edu.
/\/\/\_ Eric Van Buren, vanburen%flovax.dnet at rocdec.roc.wayne.edu
\ \ \ Immunology & Microbiology, Flow Cytometry Lab
\_^_/ Wayne State University, Detroit, Michigan
