chicken B cell differentiation

vanburen%flovax.dnet at rocdec.roc.wayne.edu vanburen%flovax.dnet at rocdec.roc.wayne.edu
Wed Mar 29 13:07:45 EST 1995

In article <9502287964.AA796438065 at internet.organo.com>, MKing at organo.com 
("King, Matt") writes:

>I am looking for any introductory info concerning flow cytometry, especially
>the use of a FACScan. I am beginning to perform research on class I and II
>antibodies and need to learn how to use the FACScan in our lab. I understand
>the priciple behind using it to a degree, but any literature you out there can
>suggest will help to strengthen my knowledge on the subject. Please help point
>me into the right direction.

Probably the best source to learn how to operate the FACScan is the 
manufacturer. Contact Becton Dickinson Immunocytometry Systems (BDIS) at:
2350 Qume Drive
San Jose, CA 95131-1807
phone: 800-448-BDIS (800-448-2347)

A good introductory text, one that is used in teaching several university 
courses on flow cytometry is:
Givan AL: Flow Cytometry: First Principles. John Wiley & Sons, Inc., New York,

A "cookbook"-style handbook of methods is:
Robinson JP (ed.): Handbook of Flow Cytometry Methods. 1990.
(The copyright is held by Purdue Research Foundation, but the handbook is 
distributed by ISAC [see next]).

The Journal of the International Society for Analytical Cytology (ISAC), called 
Cytometry, is a good resource. Send subscription inquiries to:
John Wiley & Sons, Inc., Subscription Department
9th floor
605 Third Avenue
New York, NY 10158
phone: 212-850-6543

Some more advanced texts are:
Melamed MR, Lindmo T, Mendelsohn ML (eds.): Flow Cytometry and Sorting, Second
     Edition. John Wiley & Sons, Inc., New York, 1990.
Shapiro HM: Practical Flow Cytometry, Second Edition*. Alan R. Liss, Inc.,
     New York, 1988.
*There is a newer Third Edition available now.

There is a mailing list devoted to flow cytometry. Contact Steven Kelley via 
email at:
kelley at flowcyt.cyto.purdue.edu

A few web servers dedicated mostly to flow cytometry have sprung up recently. 
The one at the Salk Institute has some tutorials/help files, as well as links 
to most of the other cytometry web servers. Open the URL:

/\/\/\_ Eric Van Buren, vanburen%flovax.dnet at rocdec.roc.wayne.edu
\ \ \   Immunology & Microbiology, Flow Cytometry Lab
 \_^_/  Wayne State University, Detroit, Michigan

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